anti slc8a1  (Boster Bio)

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Figure Lengend Snippet: Expression of distal nephron calcium transporters in WT and Clcnkb ‐deficient mice. (A–E) Gene expression analysis of the apical calcium channel Trpv5 (A), Trpv6 (B), intracellular calcium buffer Calb1 (C), and basolateral calcium extrusion transporters Slc8a1 (NCX1, D) and Atp2b4 (PMCA4, E). Slc8a1 showed no significant changes, while Trpv5, Trpv6, and Atp2b4 were significantly upregulated, and Calb1 showed a trend toward reduction in Clcnkb ‐deficient mice. (F, G) Expression of the Klotho gene ( Kl ) was significantly decreased in the knockout mice, while Egr1 was significantly upregulated. (H–K) Immunofluorescence staining of TRPV5 and Calbindin‐D28k (CaBP‐28K) in distal nephron tubules showed that expression of TRPV5 relative to tubular surface area was slightly decreased, whereas Calbindin‐D28k expression was more markedly reduced. The arrows point out dilated tubules in the Clcnkb ‐deficient mice. The scale bar represents 50 μm (H, J) or 200 μm (I, K). (L–O) Kidney lysates were subjected to immunoblotting for NCX1, PMCA4 and CaBP‐28K, and signals were densitometrically quantified. Molecular masses (in kDa) are indicated on the left. No significant differences between wildtype and Clcnkb ‐deficient mice were detected. * p < 0.05 is considered statistically significant.

Article Snippet: NCX1 , Rabbit , Alomone ANX‐011 , 1:500.

Techniques: Expressing, Gene Expression, Knock-Out, Immunofluorescence, Staining, Western Blot

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Article Title:

doi:

Figure Lengend Snippet: Antibodies.

Article Snippet: NCX1 , Rabbit , Alomone ANX‐011 , 1:500.

Techniques: Western Blot

Journal: Nature Communications

Article Title: NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease

doi: 10.1038/s41467-026-69636-1

Figure Lengend Snippet: a Representative immunofluorescence images of NCX1 (purple) co-localization with different immune cell-type markers (CD3 + , T cells, CD68 + , macrophages, CD66b + , neutrophils; CD11c + , neutrophils/dendritic cells) in distal airways and bronchioles and adjacent alveolar regions from Mixed CBE patients ( n = 5 patients). Blue, DAPI-stained nuclei. Light blue indicates colocalization of NCX1 with neutrophils. Scale bar = 30 µm. b RT-qPCR analyzes mRNA levels of NCX1 in human lung tissue from Control patients ( n = 20), emphysema patients ( n = 10), and Mixed CBE patients ( n = 20). c Western blot analysis of NCX1 protein levels in lung tissues from Control patients and Mixed CBE patients ( n = 10), normalized to β-actin and displayed relative to controls. Uncropped blots in Source Data. d , e RT-qPCR analyzes mRNA levels of MPO and NE in lung tissues from Control individuals ( n = 20), emphysema patients ( n = 10), and Mixed CBE patients ( n = 20). f–h Western blot analysis of MPO and NE expression in lung tissues from Control patients and Mixed CBE patients ( n = 10), normalized to β-actin and displayed relative to controls. Uncropped blots in Source Data. i , j Spearman’s correlation analyzes the correlation of NCX1 expression with MPO and NE levels. 95% CI: 0.5281 to 0.8724 for ( i ), 0.7029 to 1.007 for ( j ). k Representative immunofluorescence images show expression and localization of CD66b (green) and NCX1 (purple) in lung tissues of Control and Mixed CBE patients. Scale bar = 50 µm. Blue, DAPI-stained nuclei. l–n Flow cytometry identifies neutrophil numbers ( m ) and NCX1 mean fluorescence intensity ( n ) on neutrophils in human BALF ( n = 5 individuals). o Representative immunofluorescence images show expression of NCX1 in neutrophils from human BALF of Control and Mixed CBE patients. Scale bar = 2 µm. Blue, DAPI-stained nuclei. Data point represents one biologically independent replicate with three technical replicates ( b , d , e ), one biologically independent replicate with two technical replicates ( c , g , h ). Data points represent biologically independent replicates ( m , n ). Quantitative data are presented as min to max with all points mean ( b , d , e ), and as Mean ± SD ( c , g , h , m , n ). Two-sided t -test ( g , h , m , n ) and one-way ANOVA with Tukey’s multiple comparison test ( b , d , e ) were used to calculate the p- values. At least 3 times, each experiment was independently repeated with similar results. Source data are provided as a Source Data file. CBE chronic bronchitis and emphysema; MPO myeloperoxidase; NE neutrophil elastase; BALF bronchoalveolar lavage fluid; SCC-A forward scatter-area; FSC-H forward scatter-height; FSC-A side scatter-area; ns no significance.

Article Snippet: The following antibodies were used: anti–NE (Abclonal, A8953, 1:1000, rabbit), anti–MPO (Proteintech, 22225-1-AP, 1:5000, rabbit), anti–Cit-H3 (Abways, CY6587, 1:5000, rabbit), anti- Slc8a1 (Abclonal, A5583, 1:5000, rabbit), anti–β-actin (CST, 4967S, 1:1000, rabbit), and secondary antibodies (Goat Anti-Rabbit IgG, Abcam, ab6702, 1:5000).

Techniques: Immunofluorescence, Staining, Quantitative RT-PCR, Control, Western Blot, Expressing, Flow Cytometry, Fluorescence, Comparison

Journal: Nature Communications

Article Title: NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease

doi: 10.1038/s41467-026-69636-1

Figure Lengend Snippet: a Schematic diagram of CS exposure protocol in Slc8a1 f+/f+ ;Mrp8-Cre + ( Slc8a1 cKO mice) and littermate Slc8a1 f+/f+ ;Mrp8-Cre − (Control) mice. COPD is induced in Slc8a1 f +/f+ ;Mrp8-Cre + and Slc8a1 f+/f+ ;Mrp8-Cre − mice via CS exposure for 12 weeks before lung tissue collection. Slc8a1 f+/f+ ;Mrp8-Cre + and Slc8a1 f+/f+ ;Mrp8-Cre - mice were exposed under normal air in the Control group. b The pressure-volume curves were generated in the mice, presenting the mean values of volume measured under different airway pressures. c–e Pulmonary function parameters, including dynamic compliance data, FEV 50(ms) /FVC and FRC/TLC, were measured after 12-week CS exposure ( n = 6 mice). f Flow cytometry strategy identifies neutrophils from the lung tissues of Slc8a1 f+/f+ ;Mrp8-Cre + and Slc8a1 f+/f+ ;Mrp8-Cre − mice under air or CS exposure. g Flow cytometry quantification of neutrophil proportions in lung tissues ( n = 6 mice). h , i Representative H&E-stained lung sections showing alveolar destruction and airway inflammation, and corresponding mean linear intercept values ( n = 6 mice). Scale bar = 50 µm. j , k Masson’s Trichrome staining and quantification of lung collagen deposition area ( n = 6 mice). Scale bar = 50 µm. l–o Immunofluorescence and quantification for α-SMA (yellow) and SFTPC (purple) showing airway remodeling, smooth muscle hyperplasia, and loss of alveolar epithelial stem cells ( n = 6 mice). Scale bar = 20 µm. Each data point represents one biologically independent replicate with three technical replicates ( b–e , i , k , m , o ), and one biologically independent replicate ( g ). All quantitative data are presented as Mean ± SD. One-way ANOVA with Tukey’s multiple comparison test was used to calculate the p -values for all quantitative data in this figure. At least 3 times, each experiment was independently repeated with similar results. Source data are provided as a Source Data file. CS cigarette smoke; cKO conditional knockout; Cdyn dynamic compliance; FEV 50(ms) forced expiratory volume in 50 ms; FVC forced expiratory volume; FRC functional residual capacity; TLC total lung capacity; ns no significance.

Article Snippet: The following antibodies were used: anti–NE (Abclonal, A8953, 1:1000, rabbit), anti–MPO (Proteintech, 22225-1-AP, 1:5000, rabbit), anti–Cit-H3 (Abways, CY6587, 1:5000, rabbit), anti- Slc8a1 (Abclonal, A5583, 1:5000, rabbit), anti–β-actin (CST, 4967S, 1:1000, rabbit), and secondary antibodies (Goat Anti-Rabbit IgG, Abcam, ab6702, 1:5000).

Techniques: Control, Generated, Flow Cytometry, Staining, Immunofluorescence, Comparison, Knock-Out, Functional Assay

Journal: Nature Communications

Article Title: NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease

doi: 10.1038/s41467-026-69636-1

Figure Lengend Snippet: a Flow cytometry analysis shows the purity of neutrophils isolated from the bone marrow of Slc8a1 f+/f+ ;Mrp8-Cre − and Slc8a1 f+/f+ ;Mrp8-Cre + mice. b , c Flow cytometry analysis shows the phagocytosis of neutrophils to E. coli bioparticles at different time points. d In vitro phagocytosis assay. Purified neutrophils were stimulated with pHrodo Red-conjugated Escherichia coli bioparticles for 2 h and photographed by confocal microscopy. Scale bar = 5 µm. e , f Quantification of phagocytosis efficiency and index between two genotype mice ( n = 5) at 30, 60, 120 and 240 min. Each data point represents one biologically independent replicate ( e, f ). All quantitative data are presented as Mean ± SD. A two-sided t -test was used to calculate the p -values for all quantitative data. At least 3 times, each experiment was independently repeated with similar results. Source data are provided as a Source Data file. BF bright field; min minutes; ns no significance.

Article Snippet: The following antibodies were used: anti–NE (Abclonal, A8953, 1:1000, rabbit), anti–MPO (Proteintech, 22225-1-AP, 1:5000, rabbit), anti–Cit-H3 (Abways, CY6587, 1:5000, rabbit), anti- Slc8a1 (Abclonal, A5583, 1:5000, rabbit), anti–β-actin (CST, 4967S, 1:1000, rabbit), and secondary antibodies (Goat Anti-Rabbit IgG, Abcam, ab6702, 1:5000).

Techniques: Flow Cytometry, Isolation, In Vitro, Phagocytosis Assay, Purification, Confocal Microscopy

Journal: Nature Communications

Article Title: NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease

doi: 10.1038/s41467-026-69636-1

Figure Lengend Snippet: a Purified bone marrow neutrophils are stimulated by PMA (50 nM) for 4 h at 37 °C to produce NETs, and are visualized by scanning electron microscope. Scale bar = 2 µm. b NETs area is calculated as the percentage of NETs to the total area ( n = 6 mice). c ELIZA quantification of Cit-H3 levels in the cell culture supernatants of bone marrow-derived neutrophils with PMA stimulation ( n = 6 mice). d , e Representative images of immunostained MPO (green), NE (purple), Cit-H3 (yellow) co-localization and quantification of NETs area in lung sections of control individuals and Mixed CBE patients ( n = 6). Scale bar = 50 µm, 20 µm (magnified images). f , g Representative images of immunostained MPO (purple), NE (green), Cit-H3 (yellow) co-localization and quantification of NETs area in lung sections of CS-exposed Slc8a1 f+/f+ ;Mrp8-Cre − and Slc8a1 f+/f+ ;Mrp8-Cre + mice ( n = 6 mice). Scale bar = 20 µm, 10 µm (magnified images). h ELIZA detection of Cit-H3 levels in BALF of different genotype mice under air or CS exposure ( n = 6 mice). i Experimental scheme of Slc8a1 f+/f+ ;Mrp8-Cre − and Slc8a1 f+/f+ ;Mrp8-Cre + mice subjected to CS exposure for 12 weeks, followed by air exposure for 2 weeks. j FACS sorts and quantifies TRNs in the lungs of mice with different genotypes. k Quantification of TRN numbers in lungs of CS-induced mice with different genotypes after 2 weeks cessation of CS ( n = 6 mice). l–p RT-qPCR detected mRNA levels of TNF, CXCL-2, IL-6, IL-1β, and IL-17a in mouse lungs with different genotypes ( n = 6 mice) in Fig. 5i. Each data point represents one biologically independent replicate with three technical replicates ( b , c , e , g , h , l–p ). Data points in Fig. 5k represent biologically independent replicates. All quantitative data are presented as Mean ± SD. Two-sided t -test ( b , c , e , g , k , l–p ) and one-way ANOVA with Tukey’s multiple comparison test ( h ) in this figure were used to calculate the p -values. At least 3 times, each experiment was independently repeated with similar results. Source data are provided as a Source Data file. CBE chronic bronchitis and emphysema; COPD chronic obstructive pulmonary disease; PMA Phorbol 12-myristate 13-acetate; NET neutrophil extracellular trap; Cit-H3 citrullinated Histone H3; CS cigarette smoke; TRNs tissue-resident neutrophils; ns no significance.

Article Snippet: The following antibodies were used: anti–NE (Abclonal, A8953, 1:1000, rabbit), anti–MPO (Proteintech, 22225-1-AP, 1:5000, rabbit), anti–Cit-H3 (Abways, CY6587, 1:5000, rabbit), anti- Slc8a1 (Abclonal, A5583, 1:5000, rabbit), anti–β-actin (CST, 4967S, 1:1000, rabbit), and secondary antibodies (Goat Anti-Rabbit IgG, Abcam, ab6702, 1:5000).

Techniques: Purification, Microscopy, Cell Culture, Derivative Assay, Control, Quantitative RT-PCR, Comparison

Journal: Nature Communications

Article Title: NCX1 reverse mode promotes calcium-dependent Neutrophil Extracellular Trap formation and lung damage in chronic obstructive pulmonary disease

doi: 10.1038/s41467-026-69636-1

Figure Lengend Snippet: a–c RT-qPCR and WB assess NCX1 expression in neutrophils upon CSE, fMLP and LPS stimulation ( n = 3). d Immunofluorescence confirms NCX1 elevation indicated by Ly6g (green) and NCX1 (purple) in CSE-treated neutrophils ( n = 3). Blue, DAPI-stained nuclei. Scale bar = 2 µm. e Real-time Ca 2+ flux dynamics in neutrophils with or without KB-R7943 administration measured by non-invasive micro-test technique ( n = 6). Upward bars represent Ca 2+ efflux activity, and downward bars represent Ca 2+ influx. f , g Simultaneous real-time measurements of intracellular Ca 2+ (F340/380) and Na⁺ (F500/545) in neutrophils by fluorescent probes upon stimulation with CSE, PMA, or fMLP ( n = 3). h Ca 2+ influx in bone marrow-derived neutrophils from Slc8a1 f+/f+ ;Mrp8-Cre - and Slc8a1 f+/f+ ;Mrp8-Cre + mice upon CSE stimulation ( n = 3). i ELIZA detects Cit-H3 expression in the cell culture of CSE-stimulated bone marrow-derived neutrophils ( n = 6). j NETs formation in CSE-stimulated neutrophils is visualized by scanning electron microscope ( n = 3). Scale bar = 2 µm. k Effects of KB-R7943, BAPTA-AM, and A-23187 on NETs area in CSE-stimulated neutrophils. Scale bar = 2 µm. l ELIZA detects Cit-H3 expression in unstimulated neutrophils and CSE-stimulated neutrophils with KB-R7943, BAPTA-AM, and A-23187 treatment ( n = 5). Each data point represents one biologically independent replicate with 3 technical replicates ( a , b , i , l ). Data shown in Fig. 7e represent the mean of measurements (6 biological replicates). Data in Fig. 7f–h represent the mean of measurements (3 biological replicates with 3 technical replicates). All quantitative data are presented as Mean ± SD. Two-sided t -test ( i ) and one-way ANOVA with Tukey’s multiple comparison test ( a, b , l ) were used to calculate the p -values. At least 3 times, each experiment was independently repeated with similar results. Source data are provided as a Source Data file. CSE cigarette smoke extract; PMA Phorbol 12-myristate 13-acetate; fMLP N-Formylmethionyl-leucyl-phenylalanine; LPS lipopolysaccharide; Cit-H3 Citrullinated Histone H3; WT wild type; min minutes; ns no significance.

Article Snippet: The following antibodies were used: anti–NE (Abclonal, A8953, 1:1000, rabbit), anti–MPO (Proteintech, 22225-1-AP, 1:5000, rabbit), anti–Cit-H3 (Abways, CY6587, 1:5000, rabbit), anti- Slc8a1 (Abclonal, A5583, 1:5000, rabbit), anti–β-actin (CST, 4967S, 1:1000, rabbit), and secondary antibodies (Goat Anti-Rabbit IgG, Abcam, ab6702, 1:5000).

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Activity Assay, Derivative Assay, Cell Culture, Microscopy, Comparison

Journal: Journal of Advanced Research

Article Title: NCX1 interacts with TRPA1 to promote cell proliferation and tumor growth of colon cancer via disruption of calcium homeostasis

doi: 10.1016/j.jare.2025.02.031

Figure Lengend Snippet:

Article Snippet: The primary antibodies: anti-TRPA1 (1:250; Cat. No. ACC-037, Alomone Labs, Israel), anti-NCX1 (1:250; Cat. No. ANX-011, alomone labs, Israel), anti-ERK1/2 (1:1000; Cat. No. 9102, Cell Signaling Technology (CST), USA), anti-phospho-ERK1/2 (1:1000; Cat. No. 9101, CST, USA), anti-cyclin D1 (1:1000; Cat. No. MA5-16356, Thermo Fisher Scientific, USA), anti-β-catenin (1:1000; Cat. No. 8480, CST, USA), anti-phospho-β-catenin (1:1000; Cat. No. 9567, CST, USA), anti-PCNA (1:1000; Cat. No. 13110, CST, USA), anti-PI3K (1:2000; Cat. No. ABS1856, Merck, Germany), anti-AKT (1:1000; Cat. No. 4691S, CST, USA), anti-phospho-AKT (1:1000; Cat. No. 4060S, CST, USA), anti-mTOR (1:1000; Cat. No. 2983S, CST, USA), anti-phospho-mTOR (1:1000; Cat. No. 2971S, CST, USA), anti-NF-κB (1:1000; Cat. No. 8242S, CST, USA), anti-phospho-NF-κB (1:1000; Cat. No. 3033S, CST, USA), and anti-GAPDH (1:1000; Cat. No. 3683, CST, USA), the HRP-conjugated secondary antibody (Cat. No. ZB-2301, ZSGB Biotechnology, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis demonstrated that genetic susceptibility to SLC8A1 eQTL may accelerate TD progression

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: Transcriptome sequencing data analysis confirmed that genetic susceptibility to SLC8A1 eQTL contributed to TD progression

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: The experiments verified that SLC8A1 promoted progression of TD. ( A ) Photos of the Achilles tendon in control and tendinopathy groups. ( B ) H&E and Masson’s staining for control and TD tendon. Scale bar: 200 μm. ( C ) Immunofluorescence staining for SLC8A1 in control and TD tissues. Scale bar: 100 μm. ( D ) Western blotting was used to detect SLC8A1 protein levels in tendon tissues of the normal group and TD group. ( E ) qRT-PCR analysis of the mRNA expression of SLC8A1 in TD groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Quantitative RT-PCR, Expressing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis showed that genetic susceptibility to SLC8A1 eQTL was associated with reduced levels of gamma − glutamylisoleucine

Article Snippet: For immunohistochemistry, sections were blocked in solution for 2 h at room temperature and subsequently incubated overnight at 4 °C with anti-SLC8A1 antibodies (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis demonstrated that genetic susceptibility to SLC8A1 eQTL may accelerate TD progression

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques:

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: Transcriptome sequencing data analysis confirmed that genetic susceptibility to SLC8A1 eQTL contributed to TD progression

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Sequencing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: The experiments verified that SLC8A1 promoted progression of TD. ( A ) Photos of the Achilles tendon in control and tendinopathy groups. ( B ) H&E and Masson’s staining for control and TD tendon. Scale bar: 200 μm. ( C ) Immunofluorescence staining for SLC8A1 in control and TD tissues. Scale bar: 100 μm. ( D ) Western blotting was used to detect SLC8A1 protein levels in tendon tissues of the normal group and TD group. ( E ) qRT-PCR analysis of the mRNA expression of SLC8A1 in TD groups. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: Control, Staining, Immunofluorescence, Western Blot, Quantitative RT-PCR, Expressing

Journal: Molecular Cytogenetics

Article Title: SLC8A1 as a novel susceptibility gene in facilitating tendinopathy: insights into its mechanisms from Mendelian randomization and experimental validation

doi: 10.1186/s13039-025-00738-z

Figure Lengend Snippet: MR analysis showed that genetic susceptibility to SLC8A1 eQTL was associated with reduced levels of gamma − glutamylisoleucine

Article Snippet: The sections were permeabilized with 0.1% Triton X-100 in PBS for 15 min. After permeabilization, the sections were blocked using a 5% bovine serum albumin solution at ambient temperature for 1 h. The sections were incubated overnight with primary antibodies targeting SLC8A1 (1:1000, 28447-1-AP, Proteintech, Wuhan, China).

Techniques: